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PMA
产品概述：
产品参数
外观：可溶于DMSO或DMF的深红色固体
Abs = 464 nm
(before photolysis)
Abs/Em (after
photocrosslinking to nucleic acid)= 510/610 nm
贮存条件：4℃避光保存，有效期见外包装。
分子量：511
产品先容
PMA是一种高亲和性的DNA结合染料，该染料本身具有微弱的荧光，但与核酸结合后可以发出更为明亮的荧光。它尤其与双链DNA具有高亲和性。PMA不能透过细胞膜，因此只能选择性标记死细胞上暴露在外的DNA。这个特性使得PMA可以通过实时定量PCR（qPCR）的手段，广泛用于可被培养的病原细胞的筛选，因为PMA可与死细胞上的DNA牢固结合，而不能用于PCR反应的扩增。
注意事项
1、荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
2、 为了您的安全和健康，请穿实验服并戴一次性手套操作。
引用及参考文献：
引用文献
1.Detection and quantification of viable Bacillus cereus group species in milk by propidium monoazide quantitative real-time PCR
应用方向：Bacillus cereus（蜡样芽孢杆菌）
2.Persistence of Bacteroides ovatus under simulated sunlight irradiation
应用方向：Bacteroides ovales（卵巢杆菌）
3.Advantageous Direct Quantification of Viable Closely Related Probiotics inPetitSuisse Cheeses under In Vitro Gastrointestinal Conditions by Propidium Monoazide - qPCR
应用方向：Bifidobacterium animalis（动物双歧杆菌）
4.Assessing the diagnostic importance of nonviable bacterial cells in respiratory infections
应用方向：Burkholderia cepacia（赤胸鲨）
5.Impact of oxidative stress defense on bacterial survival and morphological change in Campylobacter jejuni under aerobic conditions
应用方向：Campylobacter jejuni（空肠弯曲菌）
6.Viability-PCR Shows That NAAT Detects a High Proportion of DNA from Non-Viable Chlamydia trachomatis
应用方向：Chlamydia trachomatis（沙眼衣原体 ）
7.Digestibility and prebiotic properties of potato rhamnogalacturonan I polysaccharide and its galactose-rich oligosaccharides/oligomers
应用方向：Clostridium leptum（瘦肉梭菌）
8. Survival and persistence of host-associated Bacteroidales cells and DNA in comparison with Escherichia coli and Enterococcus in freshwater sediments as quantified by PMA-qPCR and qPCR
应用方向：Enterrococcus spp（肠球菌属）
9.Detection of viable Escherichia coli O157:H7 in ground beef by propidium monoazide real-time PCR
应用方向：Escherichia coli（大肠杆菌）
10.Viability determination of Helicobacter pylori using propidium monoazide quantitative PCR
应用方向：Helicobacter pylori（幽门螺杆菌）
11.Probiotic bacteria survive in Cheddar cheese and modify populations of other lactic acid bacteria
应用方向：Lactobacillus acidophilus（嗜酸乳杆菌）
12.Fate of Viable but Nonculturable Listeria monocytogenes in Pig Manure Microcosms
应用方向：Listeria monocytogenes（单球菌）
13.A multicenter study of viable PCR using propidium monoazide to detect Legionella in water samples
应用方向：Legionella pneumophila（嗜肺军团菌）
14.Propidium monoazide and Xpert MTB/RIF to quantify Mycobacterium tuberculosis cells
应用方向：Mycobacterium tuberculosis（结核分枝杆菌）
15.Determination of the effects of medium composition on the monochloramine disinfection kinetics of Nitrosomonas europaea by the propidium monoazide quantitative PCR and Live/Dead BacLight methods
应用方向：Nitrosomonas europae（欧洲硝酸铵）
16.Study and Understanding Behavior of Alginate-Inulin Synbiotics Beads for Protection and Delivery of AntimicrobialProducing Probiotics in Colonic Simulated Conditions
应用方向：Pediococcus acidilactici（酸乳球菌）
17.Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR
应用方向：Pseudomonas aeruginosa（铜绿假单胞菌）
18.Multiplex PCR coupled with propidium monoazide for the detection of viable Cronobacter sakazakii, Bacillus cereus, and Salmonella spp. in milk and milk products
应用方向：Salmonella enterica（肠炎沙门菌）
19.Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide-Modified Quantitative PCR
应用方向：Staphylococcus aureus（金黄色葡萄球菌）
20.Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide
应用方向：Streptococcus oralis（口腔链球菌）
21. OxyRactivated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis
应用方向：Vibrio cholerae（霍乱弧菌）
22. A New Physiological Role for the DNA Molecule as a Protector against Drying Stress in Desiccation-Tolerant Microorganisms
应用方向：Microbacterium 3J1（微杆菌3J1)
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